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Quick Details

  • CAS No.: 39450-01-6
  • Other Names: Endopeptidase K
  • MF: N/A
  • EINECS No.: N/A
  • Place of Origin: Beijing, China (Mainland)
  • Purity: 85%
  • Classification: Specific Reagents
  • Brand Name: solarbio
  • Model Number: P9460
  • Activity: >30U/mg

Packaging & Delivery

Packaging Details 100mg 1g
Delivery Time Shipped in 7 days after payment
Product Description

High Active Proteinase K

Introduction:

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

 

1.Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
2.Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A.
3.Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
4.Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
5.Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

 

Storage/Stability: 2–8 °C. When stored at –20 °C, the product retains activity for at least 2 years.

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China (Mainland) | Trading Company
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